LI Shengyuan^1,2, HUANG Yongpeng², TANG Hui², WANG Chunjiang², LI Xiulan¹, CHEN Bo²

(1. School of Mechanical Engineering, Sichuan University of Science and Engineering, Yibin 644000, China;2. State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China)

Abstract: Albumin nanoparticles were prepared by reverse solvent method. The effects of different crosslinking agents were compared by single factor experiment and Z-score comprehensive evaluation method.The effects of albumin mass concentration, the volumn ratio of alcohol to water, desolubilization temperature, solvent pH, crosslinking time and other process parameters on the performance of albumin nanoparticles were studied to determine the optimal process conditions.Albumin octreotide micropowder was prepared by impregnation adsorption-freeze-drying method. The drug loading capacity and in vitro dissolution performance of the micropowder were investigated.The results show that glutaraldehyde is the preferred crosslinking agent.When the albumin mass concentration is 10 g/L, the volume ratio of alcohol to water is 2∶1, the desolubilization temperature is 20 ℃, the solvent pH is 9, and the cross-linking time is 24 h, the preparation conditions of the albumin nanoparticles are the best. The average particle size of the prepared albumin nanoparticles is (100.6±2.5) nm, the polydispersion index is 0.13±0.01, and the storage stability was good. Albumin-octreotide micropowder maintains the original spherical morphology of albumin carrier, and the continuous release time is 2 160 min, showing good in vitro release performance.

Keywords: albumin； octreotide； nanoparticle； micropowder； sustained-release properties

Received： 2022-05-09，Revised：2022-11-05，Online：2022-12-02 11:32。

Funding Project：青年科技英才基础研究项目，编号：2021C4411；四川省高校重点实验室项目，编号：2021JXY06。

First Author：李昇原(1995—)，男，硕士研究生，研究方向为微纳米材料的制备与应用。E-mail： 756622525@qq.com。

Corresponding Author:：

李秀兰(1977—)，女，副教授，博士，硕士生导师，研究方向为先进材料与表面技术。E-mail： 1853123510@qq.com。

陈博(1983—)，男，副研究员，博士，硕士生导师，研究方向为药物粉体制备与应用。E-mail： NBC_BoChen@163.com。