XIANG Yun¹,CHEN Qiang¹,XU Miao²,GAO Tianyu¹,XU Leilei¹,GUAN Jianguo¹

(1.State Key Laboratory of Advanced Technology for Materials Synthesis and Processing， International School of Materials Science and Engineering，Wuhan University of Technology，Wuhan 430070，China； 2.College of Chemistry，Central China Normal University，Wuhan 430079，China)

Abstract:Mesoporous silica nanospheres (MSNs) with enlarged pore size have been prepared by oil-water biphasic synthesis using cetyltrimethyl ammonium salts as templates and cyclohexane as oil phase reagents.Their structural and surface physicochemical properties can be regulated by changing reaction parameters and have been characterized by scanning electron microscope (SEM),transmission electron microscope (TEM),small angle X-ray diffraction (XRD) and nitrogen desorption-adsorption analysis.The results show that the porous structure of MSNs is closely related to the anions of templates.The particle sizes of MSNs increase from 50 to 100 nm with reacting again.The pore size of the as-prepared MSNs are 6.0～9.0 nm.On the research about amount of enzymes we find that the larger pore size and the better connectivity are in favor of enzyme immobilization.The amountof enzymes immobilized on MSNs reaches 230.4 mg/g that is three times higher than that on the well-known mesoporous SBA-15.Simultaneously,the enzyme activity has no significant loss after immobilization.

Keywords:mesoporous silica;nanospheres;biphasic synthesis;enzyme immobilization

中图分类号：O635.2                             文献标志码：A

文章编号：1008-5548(2020)05-0066-06

DOI:10.13732/j.issn.1008-5548.2020.05.011

收稿日期： 2020-02-25，修回日期：2020-02-28，在线出版时间：2020-07-08 09:47。

基金项目：国家自然科学基金项目，编号：21975195；国家级大学生创新创业培训计划项目，编号：201910497029。

第一作者简介：项云(1995—)，女，硕士研究生，研究方向为微纳米功能材料。E-mail：xiangyun0608@163.com。

通信作者简介：许蕾蕾(1981—)，女，博士，副研究员，博士生导师，研究方向为微纳米功能材料。E-mail：xull@whut.edu.cn。