Abstract: To study the effect of ball milling on the effective constituent dissolution of Chinese medical material Astragalus membranaceus, ball milling powders of Astragalus membranaceus with different processing time were determined by high performance liquid chromatography method，using 2 processing methods of reflux extraction and cold leaching, taking contents of astragaloside IV and calycosin-7-O-β-Dglucoside as indicators．The results show that in the reflux extraction or cold leaching conditions, compared with the powders in Chinese Pharmacopoeia, ball milling can improve the effective components of Astragalus membranaceus leaching. The content of astragaloside IV increases by 10.33% to 39.25%, calycosin-7-O-β-D-glucoside increases by 3.49% to 32.81%．The ball milling technology can effectively promote the effective constituent dissolution and dissolution rate of Astragalus membranaceus and shorten the sample preparation time.

Keywords: ball milling; Astragalus membranaceus; astragaloside IV; dissolution

中图分类号：R284.2         文献标志码：A

文章编号：1008-5548（2014）01-0013-04

DOI:10.3969/j.issn.1008-5548.2014.01.003

收稿日期：2013-08-17， 修回日期：2013-09-10，在线出版时间：2014-02-24。

基金项目：黑龙江省科技攻关计划项目，编号：WB10B112。

第一作者简介：霍金海(1981—），男，硕士，助理研究员，研究方向为中药制剂分析。 电话：0451-55665478，E-mail：jinhaihuo@126.com。

通信作者简介：王伟明(1966—），女，博士，研究员，研究方向为中药新药研发。 电话：0451-55665478，E-mail：zyyjy@163.com。